Anime characters in a laboratory with a glass jar containing a skull

a mad scientist and their assistant in an underground laboratory, the mad scientist with the crazy smile, the assistant with the creepy smile, In her hand she holds a flask containing a large, one-eyed, black, smoky creature. highly detailed, underground laboratory, torture devices, sinister experiments, lab equipment, dark lighting, eerie atmosphere, unsettling, gritty, historical background: middle ages, correct anatomy, anatomically normal hand, realistic, detailed, cinematic, award-winning cg art, 8k, hyper realistic, volumetric lighting, intricate details, unnerving, chiaroscuro lighting, extremely disturbing and gruesome, chiaroscuro lighting, dramatic shadows, muted colors, gritty, industrial, cold, sterile, claustrophobic, (best quality, 4k, 8k, highres, masterpiece:1.2), ultra-detailed, (realistic, photorealistic, photo-realistic:1.37), dark fantasy, horror

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